Journal: The EMBO Journal

Article Title: A novel human fetal lung-derived alveolar organoid model reveals mechanisms of surfactant protein C maturation relevant to interstitial lung disease

doi: 10.1038/s44318-024-00328-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Mouse anti-Mical-L1 , 1:100 , Novus , H00085377.

Techniques: Recombinant, Transduction, CRISPR, Gene Knockout, Expressing, Concentration Assay, Immunofluorescence, Western Blot, Flow Cytometry, Sequencing, Red Blood Cell Lysis, Cell Recovery, Plasmid Preparation, SYBR Green Assay, Reverse Transcription, Software, Microscopy, Magnetic Beads, Transmission Assay

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: Cytoplasmic S100A2 staining; Weak (A), Medium (B), and Strong (C) expression in CRC TMAs.

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques: Staining, Expressing

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: Kaplan-Meier survival analysis based on cytoplasmic S100A2 expression for cancer specific survival (CSS) in CRC patients.

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: The relationship between cytoplasmic S100A2 status and clinical characteristic in CRC patients

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques: Expressing

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: (A) Hierarchical clustering of the top 50 most differentially expressed genes between low (Grey) and high (Amber) S100A2 protein expression. (B) Volcano plot showing the distribution of gene expression fold changes and p values between patients with high and low S100A2; Red means up-regulated in cases with low S100A2 and blue means up-regulated in cases with high S100A2.

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques: Expressing, Gene Expression

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: (A) Enrichment analysis by tumour with low and high cytoplasmic S100A2 in CRC from GSEA software (B) Enrichment plots using gene set immune signature (c7.all.v2022.1.Hs.symbols.gmt database).

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques: Software

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: Bar charts showing the percentage of (A) CD3FOXP3 and (B) CD66b and (C) CD68 and (D) CD163 relative to S100A2 status in CRC.

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques:

Journal: Journal of Cancer

Article Title: Protein expression of S100A2 reveals it association with patient prognosis and immune infiltration profile in colorectal cancer

doi: 10.7150/jca.83910

Figure Lengend Snippet: Proposed mechanism of S100A2 for regulating P53 to induce tumour-related signalling, created with BioRender.

Article Snippet: S100A2 293T cell transient overexpressed lysate (H00006273-T01, Novus biologicals) was loaded into a 4-12% sodium dodecyl sulfate-polyacrylamide gel and separated by electrophoresis (SDS-PAGE).

Techniques:

Journal: JCI Insight

Article Title: Unbiased cleavage site prediction uncovers viral antagonism of host innate immunity by SARS-CoV-2 3C-like protease

doi: 10.1172/jci.insight.185739

Figure Lengend Snippet: ( A ) Identification of COVID-19 GWAS hits adjacent (± 150bp) to genomic locations of SARS-CoV-2 3CLPro cut sites. Log 10 Sarsport score shown on x axis, Log 10 P value of GWAS hits shown on y axis. Highlighted are clusters of high probability SNPs at high-scoring cut sites. ( B ) OAS1 isoforms COVID-19 GWAS SNPs. The p42 (major allele) isoform and p46 (minor alleles) are determine by a single SNP, rs10774671. A high scoring cut-site (Q384) is located 12 aa upstream of the C-terminus of the p46 OAS1 isoform, which encodes a prenylation site. ( C ) AlphaFold structure of full-length p46 OAS1 with predicted cut-site highlighted. ( D ) Western blot of 293T cells overexpressing p46 OAS1 and C145A (catalytically inactive) or active 3CLPro. Full-length (46 kDa) and cleaved product (44 kDa) highlighted. Strep-Tag and HSP90 controls were run on a separate gel from OAS1 staining. ( E ) Immunocytochemistry of 293T cells overexpressing p46 OAS1 and C145A or 3CLPro. Costaining with WGA shown to mark subcellular membrane structures. Scale bar: 10 μm. ( F ) Colocalization quantification of WGA and OAS1 from E . In total, 60–80 individual OAS1 + /3CLPro + or OAS1 + /C145A + cells were traced and colocalization quantified by Pearson’s R value of linear using the Coloc2 ImageJ plugin (NIH). Comparison statistics of R values quantified by 2-tailed Student’s t test. ( G ) Immunocytochemistry of ACE2-293T cells expressing V5 tagged p46 OAS. Costaining of V5 to mark OAS1 with WGA shown to mark subcellular membrane structures. Scale bar: 5 μm. ( H ) Colocalization quantification of WGA and OAS1 from control or COVID infected cells in G . Ten to 18 individual cells were traced and colocalization of WGA and OAS1 quantified by Pearson’s R value of linear using the Coloc2 ImageJ plugin. Comparison statistics of R values quantified by 2-tailed Student’s t test.

Article Snippet: For 293T overexpression assays, commercially available 293T (ATCC, catalog CRL 3216) or ACE2-293T (Takara, catalog 631289) were cultured according to manufacturer’s protocol.

Techniques: Western Blot, Strep-tag, Staining, Immunocytochemistry, Membrane, Comparison, Expressing, Control, Infection